Search results for "Coomassie Brilliant Blue"

showing 2 items of 2 documents

Sponge aggregation factor and sponge hemagglutinin: possible relationships between two different molecules.

1979

Abstract A lectin from the marine sponge GEODIA CYDONIUM was isolated and characterized. GEODIA lectin (GL) agglutinates human red blood cells irrespective of the ABO blood group and precipitates with a variety of D -galactose containing glycosubstances, i.e. certain snail galactans, bovine erythrocyte glycoprotein and PNEUMOCOCCUS type XIV polysaccharide. The only simple sugars inhibiting the GL-mediated hemagglutination were lactose and n -acetyl- D -galactosamine. GL was purified by affinity chromatography on Sepharose 4B almost to homogeneity as tested by polyacrylamide disc gel electrophoresis. Positive staining of the lectin band with Coomassie brilliant blue and PAS suggest that GL i…

ImmunodiffusionHot TemperatureImmunologyOligosaccharidesBiologySepharosechemistry.chemical_compoundAffinity chromatographyLectinsAnimalsGeodiaCell AggregationCoomassie Brilliant BlueLectinHemagglutininHemagglutination Inhibition Testsbiology.organism_classificationCell aggregationPoriferaMolecular WeightHemagglutininschemistryBiochemistryAgglutininsGalactosebiology.proteinChromatography GelElectrophoresis Polyacrylamide GelDevelopmental BiologyDevelopmental and comparative immunology
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Protein Quantitation: Bradford Method

2002

Protein quantitation according to the Bradford method. Keywords: protein quantitation; bradford; coomassie brilliant blue; calorimetric quantification

chemistry.chemical_compoundfluids and secretionsChromatographychemistryCoomassie Brilliant Blueparasitic diseasesQuantitative proteomicsequipment and supplieshuman activitiesBradford protein assayeLS
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